Background: Root cause analysis (RCA) is a structured investigation methodology aimed at identifying systems factors to prevent recurrence of incidents. To enhance staff's knowledge and skills, a hybrid RCA training course was conducted in February 2021. Overseas instructors conducted training online and local participants attended the training together physically with onsite facilitator support. This study aimed at understanding the experiences of trainees who have undergone the training, evaluated its effectiveness and identified opportunities to enhance RCA training quality in the future.
Methods: A qualitative study using virtual synchronous focus group interviews was conducted. Purposive sampling was adopted to invite all trainees from the RCA training course to join. A semistructured interview was used to guide the study participants to share their experiences. All groups were audio-recorded, transcribed verbatim and anonymised for data analysis.
Results: Overall, 6 focus groups with 19 participants were held between July and November 2021. Five key themes were identified including: (1) training contents, (2) perceptions of RCA, (3) challenges in RCA, (4) hybrid training and (5) future perspectives. Participants felt the RCA training was useful and broadened their understanding in incident investigation. More in-depth training in interviewing skills, report writing with practical sessions could further enhance their competencies in RCA. Participants accepted the use of hybrid online-offline training well. Most participants would welcome an independent organisation to conduct RCA as findings would be more objective and recommendations more effective.
Conclusions: This study provided an evaluation on the effectiveness of a hybrid RCA training course. Healthcare and training organisations can consider this training mode as it could reduce the cost of training and enhance flexibility in course arrangement while preserving quality and effectiveness. Virtual focus groups to interview participants were found to be convenient as it minimised travelling time and onsite arrangement while maintaining the quality of discussion.
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http://dx.doi.org/10.1136/bmjoq-2022-002153 | DOI Listing |
Small Methods
January 2025
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.
Rolling circle amplification (RCA)-derived ultra-long DNA is highly attractive and versatile because of its diverse functionalities conferred by repeated DNA nanostructures. However, magnesium pyrophosphate (MgPPi) crystals, as byproducts of RCA, electrostatically interact with the DNA to form DNA microhybrids and hamper its broad bioapplications, as its large size is unfavorable for cellular uptake and decreases the density of functional DNA nanostructures. In this study, finely tuned synthesis strategies are developed to condense the microhybrids and replace non-functional MgPPi crystals with various functional metal nanostructures by reducing metal ions.
View Article and Find Full Text PDFAnal Chem
January 2025
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
Herein, a supramolecular DNA nanodevice was formed via the rolling circle amplification (RCA) and hybridization chain reaction (HCR) cascade reaction on a tetrahedral DNA nanostructure (TDN) to achieve simultaneous sensitive detection and intracellular imaging of dual-miRNAs related to liver cancer. The supramolecular DNA nanodevice effectively addressed the limitations of low probe loading capacity in traditional TDN nanodevices by enriching plenty of signal probes around a single TDN, significantly enhancing the fluorescence signal. Impressively, the supramolecular DNA nanodevice with a TDN fulcrum and dense DNA structure imparted the nanodevice with strong rigidity, ensuring the stability of the signal probes to decrease aggregation quenching for further increasing the fluorescence response.
View Article and Find Full Text PDFFront Cardiovasc Med
December 2024
Cardiovascular Medicine Department, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Objective: To explore whether radiomics analysis of pericoronary adipose tissue (PCAT) captured by coronary computed tomography angiography (CCTA) could discriminate unstable angina (UA) from stable angina (SA).
Methods: In this single-center retrospective case-control study, coronary CT images and clinical data from 240 angina patients were collected and analyzed. Patients with unstable angina ( = 120) were well-matched with those having stable angina ( = 120).
Sports Med
December 2024
Department of Sport, Exercise and Rehabilitation, Northumbria University, Newcastle Upon Tyne, UK.
Background: To combat the high prevalence of physical inactivity among children, there is an urgent need to develop and implement real-world interventions and policies that promote physical activity (PA) and reduce sedentary behaviour (SB). To inform policy makers, the current body of evidence for children's PA/SB interventions needs to be translated.
Objectives: The current systematic review and meta-analysis aimed to identify modifiable determinants of device-measured PA and SB targeted in available intervention studies with randomized controlled trial (RCT) and controlled trial (CT) designs in children and early adolescents (5-12 years) and to quantify the effects of the interventions within their respective settings on the determinants of PA/SB and the outcomes PA and SB.
Talanta
December 2024
Institute for Agricultural Product Quality Safety and Testing Technology, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China. Electronic address:
This paper presents a self-powered biosensing platform based on graphdiyne@Au (2D GDY@Au) nanoparticles and rolling circle-hybridization chain (RC-HC) dual linear cascade amplification technology, which significantly enhances target recognition and signal amplification efficiency for miRNA-141. Specifically, the target on bioanode outputs a large amount of single-stranded DNA (T1) through the strand displacement amplification (SDA) mechanism. This efficient target recycling process triggers RC-HC dual linear cascade reaction.
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