Effectiveness of final cavity disinfectant terminalia chebula, malachite, and indocyanine green, against E. Faecalis and on the bond interface of fiber post to radicular dentin.

Aims: Evaluation of antimicrobial efficacy of contemporary disinfection Malachite green (MG), Terminalia chebula (T. chebula), and Indocyanine green (ICG)) against E. faecalis and their effect on push-out bond strength (PBS) of glass fiber post (GFP) bonded to root dentin in comparison to conventional irrigation used (Sodium hypochlorite (NaOCl) + Ethylene diamine tetraacetic acid (17% EDTA).

Materials And Methods: Root canal treatment was initiated on sixty human single-rooted premolars and working length was established. The canals were enlarged using ProTaper universal system till F3. The prepared canals were obturated with Gutta-percha (GP) and AH Plus sealer followed by post-space preparation. Twenty canals contained a standard strain of E. faecalis (n=5 in each group) cultured overnight at 37 °C in BHI broth. All the samples were randomly allocated into four groups based on post-space irrigation. (n = 10) Group 1: 5.25% NaOCl+17% EDTA (Control), Group 2: 5.25% NaOCl + ICG, Group 3: 5.25% NaOCl + MG and Group 4: 5.25% NaOCl + T. chebula. Survival rates of E. faecalis were evaluated on the twenty specimens. GFP was placed on the remaining samples using self-etch dual-cure pastes and sectioning was performed in 1 mm thick slices. PBS and failure mode were analyzed using a universal testing machine and stereomicroscope. One-way analysis of variance (ANOVA) and Tukey multiple comparison t-tests were used for data analysis. (p = 0.05).

Results: 5.25% NaOCl+17% EDTA demonstrated (0.11±0.01 CFU/mL) the lowest survival rate of E. faecalis. However, Group 2 (5.25% NaOCl + ICG) exhibited the highest survival rate (0.52 ± 0.10). Furthermore, the coronal third of group 4 (5.25% NaOCl + T. chebula) (8.71±0.25 MPa) specimens demonstrated the highest PBS of GFP. However, an apical section of group 2 samples (5.25% NaOCl + ICG) (1.21±0.71 MPa) displayed the lowest outcome of bond integrity.

Conclusion: Terminalia chebula when used as a final canal disinfectant seems to be promising in improving canal sterility and the bond strength of GFP to the root dentin.