Objectives: Patients with urethral stricture due to any type of trauma, hypospadias or gender dysphoria suffer immensely from impaired capacity to urinate and are in need of a new functional urethra. Tissue engineering with decellularization of a donated organ recellularized with cells from the recipient patient has emerged as a promising alternative of advanced therapy medicinal products. The aim of this pilot study was to develop an ovine model of urethral transplantation and to produce an individualized urethra graft to show proof of function .
Methods: Donated urethras from ram abattoir waste were decellularized and further recellularized with autologous buccal mucosa epithelial cells excised from the recipient ram and expanded . The individualized urethral grafts were implanted by reconstructive surgery in rams replacing 2.5 ± 0.5 cm of the native penile urethra.
Results: After surgery optimization, three ram had the tissue engineered urethra implanted for one month and two out of three showed a partially regenerated epithelium.
Conclusions: Further adjustments of the model are needed to achieve a satisfactory proof-of-concept; however, we interpret these findings as a proof of principle and a possible path to develop a functional tissue engineered urethral graft with de- and recellularization and regeneration after transplantation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1515/bmt-2022-0386 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Background: Tooth autotransplantation is a reliable procedure with high long-term success and survival rates, showing many advantages over dental implants, particularly for young patients in craniofacial development. Nonetheless, insufficient bone availability at the recipient site is a key limitation. This study aims to establish the feasibility of tooth autotransplantation combined with staged or simultaneous bone regeneration to address complex cases.
View Article and Find Full Text PDFTissue Engineering 3D-Printed Scaffold Using Allograft/Alginate/Gelatin Hydrogels Coated With Platelet-Rich Fibrin or Adipose Stromal Vascular Fraction Induces Osteogenesis In Vitro.
J Cell Physiol
December 2024
Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Incorporating autologous patient-derived products has become imperative to enhance the continually improving outcomes in bone tissue engineering. With this objective in mind, this study aimed to evaluate the osteogenic potential of 3D-printed allograft-alginate-gelatin scaffolds coated with stromal vascular fraction (SVF) and platelet-rich fibrin (PRF). The primary goal was to develop a tissue-engineered construct capable of facilitating efficient bone regeneration through the utilization of biomaterials with advantageous properties and patient-derived products.
View Article and Find Full Text PDFExtended Platelet-Rich Fibrin as a Membrane for Lateral Window Sinus Lifts: A Case Series.
Clin Implant Dent Relat Res
December 2024
Department of Periodontology, University of Bern, Bern, Switzerland.
Introduction: Platelet-rich fibrin (PRF) is being increasingly utilized in surgical procedures due to various improvements in clinical outcomes. More recently, a heating process to denature albumin in the platelet poor plasma (PPP) layer has been shown to extend the resorption time of PRF from a typical 2-week period to 4-6 months. Because of its > 4 month resorption properties, extended PRF (e-PRF) membranes have been used in dentistry as an alternative to collagen membranes in alveolar ridge preservations, ridge augmentations, soft tissue grafting, and as a barrier membrane in lateral sinus grafting procedures.
View Article and Find Full Text PDFMagnesium Membrane Shield Technique for Alveolar Ridge Preservation: Step-by-Step Representative Case Report of Buccal Bone Wall Dehiscence with Clinical and Histological Evaluations.
Biomedicines
November 2024
Department of Anatomy, Histology, Embriology, Pathology Anatomy and Pathology Histology, Faculty of Dental Medicine and Health Osijek, J.J. Strossmayer University of Osijek, 31000 Osijek, Croatia.
Background/objectives: Despite the increased use of new resorbable magnesium membranes, there are no reported cases or studies on the use of resorbable magnesium membranes in combination with bone grafts for alveolar ridge preservation (ARP) in cases with severe buccal bone wall dehiscence. This case report aimed to evaluate the effectiveness of the magnesium membrane shield technique in conjunction with bone grafting for ARP, assessing both clinical outcomes and histological bone regeneration.
Methods: A 44-year-old female patient presented with a vertical fracture on tooth 24 (FDI Notation System) accompanied with complete destruction of the buccal bone wall.
Comparative evaluation of autologous dental bone powder and deproteinized bovine bone mineral allografts for oral implant bone deficits.
Clin Oral Investig
November 2024
Department of Stomatology, First Hospital of Handan City, Handan, Hebei Province, China.
Article Synopsis
- The study aimed to evaluate how well autologous dental bone powder grafts perform compared to deproteinized bovine bone mineral (DBBM) allografts in patients with oral implant bone deficiency over a two-year period.
- In the experimental group receiving autologous grafts, faster wound healing and a higher rate of full healing (Grade I) were observed compared to the control group, which used DBBM allografts.
- While both groups experienced a decrease in alveolar bone width three months after extraction, the experimental group showed maintained or increased bone height compared to the control group, indicating better overall bone preservation.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!