AI Article Synopsis
- - The study focused on isolating a lytic phage (vB_PaeP_ASP23) from mink farm sewage, studying its genome, and evaluating its effectiveness against certain bacteria in minks.
- - Phage ASP23 has a 10-minute latent period, a burst size of 140 pfu per infected cell, and its complete genome consists of 42,735 base pairs of double-stranded DNA with 54 predicted open reading frames.
- - The phage demonstrated significant antibacterial activity by reducing bacterial counts in minks and showed potential for developing new antibacterial agents, particularly using its lysin and holin in combating specific bacteria strains.
Article Abstract
In this study, we isolated a lytic phage (vB_PaeP_ASP23) from the sewage of a mink farm, characterized its complete genome and analyzed the function of its putative and . Morphological characterization and genome annotation showed that phage ASP23 belonged to the family genus , and it had a latent period of 10 min and a burst size of 140 pfu/infected cell. In minks challenged with , phage ASP23 significantly reduced bacterial counts in the liver, lung, and blood. The whole-genome sequencing showed that its genome was a 42,735-bp linear and double-stranded DNA (dsDNA), with a G + C content of 62.15%. Its genome contained 54 predicted open reading frames (ORFs), 25 of which had known functions. The lysin of phage ASP23 (LysASP), in combination with EDTA, showed high lytic activity against L64. The holin of phage ASP23 was synthesized by M13 phage display technology, to produce recombinant phages (HolASP). Though HolASP exhibited a narrow lytic spectrum, it was effective against and . However, these two bacteria were insensitive to LysASP. The findings highlight the potential of phage ASP23 to be used in the development of new antibacterial agents.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045481 | PMC |
| http://dx.doi.org/10.3389/fmicb.2023.1093668 | DOI Listing |
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Article Synopsis
- - The study focused on isolating a lytic phage (vB_PaeP_ASP23) from mink farm sewage, studying its genome, and evaluating its effectiveness against certain bacteria in minks.
- - Phage ASP23 has a 10-minute latent period, a burst size of 140 pfu per infected cell, and its complete genome consists of 42,735 base pairs of double-stranded DNA with 54 predicted open reading frames.
- - The phage demonstrated significant antibacterial activity by reducing bacterial counts in minks and showed potential for developing new antibacterial agents, particularly using its lysin and holin in combating specific bacteria strains.
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