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Application of a core genome sequence typing (cgMLST) pipeline for surveillance of in China. | LitMetric
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Application of a core genome sequence typing (cgMLST) pipeline for surveillance of in China.

Yuan Yuan Wang Lu Xie Wen Zhu Zhang Xiao Li Du Wen Ge Li Lu Lu Bia Zhi Gang Cui Yuan Wu Jin Xing Lu

Front Cell Infect Microbiol

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Published: March 2023

AI Article Synopsis

  • The text discusses a nosocomial bacterial pathogen responsible for antibiotic-associated diarrhea, highlighting the role of exotoxins and methods for genetic typing such as MLST and whole genome sequencing (WGS).
  • A study analyzed 699 genome sequences of distinct strains to establish a core gene set and develop a core genome multilocus sequence typing (cgMLST) scheme for phylogenetic analysis.
  • The findings categorized the bacterial isolates into five clades and successfully tracked an outbreak, suggesting a valuable surveillance system for this pathogen in China.

Article Abstract

Introduction: is a nosocomial bacterial pathogen that causes antibiotic-associated diarrhea mediated by cellular exotoxins secreted into the intestine during bacterial growth. Multilocus sequence typing (MLST) and PCR ribotyping are the main molecular typing for . Whole genome sequencing (WGS) core genome multilocus sequence typing (cgMLST) was developed for genetic evolution and outbreak investigation of with higher precision and accuracy.

Methods: A total of 699 whole (complete and draft) genome sequences of distinct strains were used in this study to identify core gene set (2469 core genes) and the cgMLST scheme for the phylogeny analysis of . This cgMLST pipeline was then carried the Chinese Pathogen Identification Net (China PIN) for surveillance of in China. Within the China PIN, 195 WGS of and an outbreak of CDI with 12 WGS of were used to evaluate the cgMLST pipeline.

Results: The result displayed that mostly tested isolates could be successfully divided into 5 classic clades and the outbreak event was also successfully identified.

Discussion: The results are meaningful and offer a practicable pipeline for a national-wide surveillance of in China.

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 Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10040748PMC
http://dx.doi.org/10.3389/fcimb.2023.1109153DOI Listing

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