Characterization of the NAC Transcription Factor in Passion Fruit () and Functional Identification of in Cold Stress.

The NAC (NAM, ATAF and CUC) gene family plays an important role in plant development and abiotic stress response. However, up to now, the identification and research of the NAC () family members of passion fruit are still lacking. In this study, 25 were identified from the passion fruit genome, and their functions under abiotic stress and at different fruit-ripening stages were analyzed. Furthermore, we analyzed the transcriptome sequencing results of under four various abiotic stresses (drought, salt, cold and high temperature) and three different fruit-ripening stages, and verified the expression results of some genes by qRT-PCR. Additionally, tissue-specific analysis showed that most were mainly expressed in flowers. In particular, was induced by four various abiotic stresses. At present, low temperatures have seriously endangered the development of passion fruit cultivation. Therefore, was transformed into tobacco, yeast and Arabidopsis to study their function of resisting low temperature. The results show that responded to cold stress significantly in tobacco and Arabidopsis, and could improve the low temperature tolerance of yeast. This study not only improved the understanding of the gene family characteristics and evolution, but also provided new insights into the regulation of the gene at different stages of fruit maturation and abiotic stresses.