AI Article Synopsis
- Overexpression of the Glu-1Bx7 allele enhances dough strength and overall wheat quality, but it's rare in many varieties.
- Four cultivars containing the 1Bx7 allele were crossbred to create Near Isogenic Lines (NILs), including Chisholm and Keumkang, both of which also have 1Bx7.
- Analysis showed that the NILs had significantly higher protein content (12.94%) and SDS-sedimentation values compared to the parental varieties, suggesting that crossbreeding these cultivars can improve domestic wheat quality.
Article Abstract
Overexpression of Glu-1Bx7 via allele 1Bx7 significantly contributes to high dough strength in some wheat varieties and is useful for improving wheat quality. However, the proportion of wheat varieties containing Bx7 is quite low. In this study, four cultivars containing 1Bx7 were selected, and among the selected varieties, Chisholm (1Ax2*, 1Bx7 + 1By8*, and 1Dx5 + 1Dx10) was crossed with Keumkang, a wheat variety that contains 1Bx7 (1Ax2*, 1Bx7 + 1By8, and 1Dx5 + 1Dx10). SDS-PAGE and UPLC analyses showed that the expression of the high-molecular-weight glutenin subunit (HMW-GS) 1Bx7 was significantly higher in NILs (1Ax2*, 1Bx7 + 1By8*, and 1Dx5 + 1Dx10) compared with that in Keumkang. Wheat quality was analyzed with near infrared reflectance spectroscopy by measuring the protein content and SDS-sedimentation of NILs. The protein content of NILs (12.94%) was 21.65% higher than that of Chisholm (10.63%) and 4.54% higher than that of Keumkang (12.37%). In addition, the SDS-sedimentation value of NILs (44.29 mL) was 14.97% and 16.44% higher than that of Keumkang (38.52 mL) and Chisholm (38.03 mL), respectively. This study predicts that the quality of domestic wheat can be improved by crossbreeding with 1Bx7-containing cultivars.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10051937 | PMC |
| http://dx.doi.org/10.3390/plants12061244 | DOI Listing |
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Article Synopsis
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