The () gene family is composed of two members ( and ) with high-sequence homology that encode transcription factors involved in the modulation of virulence in the species complex (FOSC). While is a multicopy gene exclusive of highly virulent strains of FOSC and is located in the accessory genome, is a single-copy gene, located in the core genome, and well-conserved in all filamentous ascomycete fungi, except yeast. The involvement of in the colonization of the vascular system and regulation of the expression of SIX effectors has been stablished. To address the role of , we generated and characterized mutants defective in in a f. sp. weakly virulent strain and analyzed them together with the equivalent mutants formerly obtained in a highly virulent strain. The results obtained highlight a role for as a negative regulator of the production of macroconidia and demonstrate that it is required for full virulence and the positive regulation of SIX effectors. In addition, gene expression analyses provided compelling evidence that is involved in the regulation of hydrophobins likely required for plant colonization.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054582 | PMC |
| http://dx.doi.org/10.3390/pathogens12030380 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Isolation and Identification of Apiospora intestini from Hedera nepalensis Leaf Blight and Determination of Antagonism of Phomopsis sp.
Curr Microbiol
January 2025
College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming, 650224, China.
In order to identify the pathogen responsible for Hedera nepalensis leaf blight and investigate effective biocontrol strategies, samples were collected from 10 significantly infected areas at Southwest Forestry University; four to six infected leaves were gathered from each area, followed by the isolation and purification of strains from the infected plant leaves using tissue isolation and hyphae-purification techniques. We conducted an examination of the biological characteristics and compared the inhibitory effects of different concentrations of Phomopsis sp. (50%, 25%, 16.
View Article and Find Full Text PDFAn augmented transformer model trained on protein family specific variant data leads to improved prediction of variants of uncertain significance.
Hum Genet
January 2025
TCS Research, Tata Consultancy Services, Hyderabad, India.
Variants of uncertain significance (VUS) represent variants that lack sufficient evidence to be confidently associated with a disease, thus posing a challenge in the interpretation of genetic testing results. Here we report an improved method for predicting the VUS of Arylsulfatase A (ARSA) gene as part of the Critical Assessment of Genome Interpretation challenge (CAGI6). Our method uses a transfer learning approach that leverages a pre-trained protein language model to predict the impact of mutations on the activity of the ARSA enzyme, whose deficiency is known to cause a rare genetic disorder, metachromatic leukodystrophy.
View Article and Find Full Text PDFBacteria can change morphology in response to stressors and changes in their environment, including infection of a host. We previously identified the bacterial species, , which uses nutrient-induced filamentation as a novel mechanism for cell-to-cell spreading in the intestinal epithelial cells of a nematode host. To further investigate the conservation of nutrient-induced filamentation in Bordetellae, we utilized the turkey-infecting species which filaments in vitro when switched from a standard growth media to an enriched media.
View Article and Find Full Text PDFTandem repeats are a highly polymorphic class of genomic variation that play causal roles in rare diseases but are notoriously difficult to sequence using short-read techniques . Most previous studies profiling tandem repeats genome-wide have reduced the description of each locus to the singular value of the length of the entire repetitive locus . Here we introduce a comprehensive database of 3.
View Article and Find Full Text PDFMolecular characterization and genome sequencing of selected highly resistant clinical isolates of and its association with the clustered regularly interspaced palindromic repeat/Cas system.
Heliyon
January 2025
Department of Microbiology, College of Medicine, University of Anbar, Anbar Governorate, Ramadi, Iraq.
The presence of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system in the superbug presents a unique opportunity to precisely target and edit bacterial genomes to modify their drug resistance. The objective was to detect the prevalence of CRISPR in extensively and pan-drug-resistant and to determine the utility of whole-genome sequencing (WGS) for the analysis of the entire genome for such strains. The antimicrobial susceptibilities of one hundred isolates were assessed using the antibiotic susceptibility test (AST) card of the VITEK system.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!