MSF Enhances Human Antimicrobial Peptide -Defensin (HBD2 and HBD3) Expression and Attenuates Inflammation via the NF-B and p38 Signaling Pathways.

Both defensin and inflammation are part of the human innate immune system that responds rapidly to pathogens. The combination of defensins with pro- or anti-inflammatory effects can be a potential research direction for the treatment of infection by pathogens. This study aimed to identify whether MSF (Miracle Synergy material made using ) a probiotic lysate of extracts fermented with K8, activates the expression of human -defensin (HBD2 and HBD3) to protect the host against pathogens and inhibit inflammation caused by , in vitro with Western blot analysis, qRT-PCR and in vivo studies with a mouse model were used to evaluate the effects of MSF. The MSF treatment induced HBD2 and HBD3 expression via the p38 and NF-B pathways. Furthermore, MSF treatment significantly reduced the expression of pro-inflammatory cytokines (TNF-, IL-1, IL-6, and IL-8), also through p38 and NF-B in -induced inflammatory condition. MSF treatment remarkably reduced erythema in mice ears caused by the injection of , while K8 lysate treatment did not initiate a strong recovery. Taken together, MSF induced the expression of HBD2 and HDB3 and activated anti-inflammatory activity more than the probiotic lysates of K8. These findings show that MSF is a potential defensin inducer and anti-inflammatory agent.