AI Article Synopsis

  • Deep-sea microorganisms adapt to high hydrostatic pressure through modifications in their respiratory components, but their ATP production strategies are less understood.
  • The study focused on the deep-sea bacterium SS9, revealing that it shows better growth and ATP production in minimal glucose medium compared to a complex medium under varying pressure conditions.
  • Researchers discovered that SS9 has two ATPase systems, with ATPase-I dominating in complex medium and ATPase-II being more prominent in minimal medium, indicating a functional relationship between these systems when under pressure.

Article Abstract

Alteration of respiratory components as a function of pressure is a common strategy developed in deep-sea microorganisms, presumably to adapt to high hydrostatic pressure (HHP). While the electron transport chain and terminal reductases have been extensively studied in deep-sea bacteria, little is known about their adaptations for ATP generation. In this study, we showed that the deep-sea bacterium SS9 exhibits a more pronounced piezophilic phenotype when grown in minimal medium supplemented with glucose (MG) than in the routinely used MB2216 complex medium. The intracellular ATP level varied with pressure, but with opposite trends in the two culture media. Between the two ATPase systems encoded in SS9, ATPase-I played a dominant role when cultivated in MB2216, whereas ATPase-II was more abundant in the MG medium, especially at elevated pressure when cells had the lowest ATP level among all conditions tested. Further analyses of the Δ, Δ1 and Δ2 mutants showed that disrupting ATPase-I induced expression of ATPase-II and that the two systems are functionally redundant in MB2216. Collectively, we provide the first examination of the differences and relationships between two ATPase systems in a piezophilic bacterium, and expanded our understanding of the involvement of energy metabolism in pressure adaptation.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10054830PMC
http://dx.doi.org/10.3390/microorganisms11030637DOI Listing

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Article Synopsis
  • Deep-sea microorganisms adapt to high hydrostatic pressure through modifications in their respiratory components, but their ATP production strategies are less understood.
  • The study focused on the deep-sea bacterium SS9, revealing that it shows better growth and ATP production in minimal glucose medium compared to a complex medium under varying pressure conditions.
  • Researchers discovered that SS9 has two ATPase systems, with ATPase-I dominating in complex medium and ATPase-II being more prominent in minimal medium, indicating a functional relationship between these systems when under pressure.
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