AI Article Synopsis

  • Paracoccidioidomycosis (PCM) is the most common systemic fungal infection in Latin America, previously thought to be caused by a single species, but has since been updated to include multiple cryptic species, including S1, PS2, PS3, and PS4.
  • Researchers at the Hospital of Medical School of Botucatu-UNESP aimed to analyze biopsy samples from PCM patients to understand the distribution of these species in the Botucatu region, an area known for its PCM prevalence.
  • Using molecular techniques like DNA extraction and PCR, they found that 100% of the positive samples corresponded to the S1 species, highlighting its dominance in the São Paulo State region.

Article Abstract

Paracoccidioidomycosis (PCM) is the main and most prevalent systemic mycosis in Latin America, that until recently, it was believed to be caused only by (). In 2006, researchers described three cryptic species: S1, PS2, PS3, and later, another one, PS4. In 2009, (Pb01-like) was described, and in 2017, a new nomenclature was proposed for the different agents: (S1), (PS2), (PS3), and (PS4). These species are not uniformly distributed throughout Latin America and, knowing that more than one cryptic species could coexist in some regions, we aimed to identify those species in patients' biopsy samples for a better understanding of the distribution and occurrence of these recently described species in Botucatu region. The Hospital of Medical School of Botucatu-UNESP, which is a PCM study pole, is located in São Paulo State mid-west region and is classified as a PCM endemic area. Genotyping analyses of clinical specimens from these patients that have been diagnosed and treated in our Hospital could favor a possible correlation between genetic groups and mycological and clinical characteristics. For this, molecular techniques to differentiate species in these biopsies, such as DNA extraction, PCR, and sequencing of three target genes (ITS, CHS2, and ARF) were conducted. All the sequences were analyzed at BLAST to testify the presence of . The phylogenetic trees were constructed using Mega 7.0 software and showed that 100% of our positive samples were from S1 cryptic species, therefore . This is important data, demonstrating the predominance of this species in the São Paulo State region.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055015PMC
http://dx.doi.org/10.3390/microorganisms11030562DOI Listing

Publication Analysis

Top Keywords

cryptic species
12
species
9
latin america
8
ps2 ps3
8
ps3 ps4
8
são paulo
8
paulo state
8
molecular phylogenetic
4
phylogenetic analysis
4
analysis species
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!