AI Article Synopsis
- - The study focused on evaluating the allergenic properties of Blo t 2, a specific allergen from house dust mites, using both human and mouse models to assess its effects.
- - Results showed that sensitivity to Blo t 2 was comparable to another allergen, Blo t 21, but higher than Der p 2, with most patients having a low-intensity allergic response.
- - Immunization of mice with Blo t 2 led to significant allergic reactions, including skin inflammation and lung issues, confirming its allergenic activity and importance in clinical settings.
Article Abstract
Only few allergens derived from house dust mite (HDM) species have been evaluated in terms of their potential to induce allergic inflammation. In this study, we aimed to evaluate different aspects of the allergenicity and allergenic activity of Blo t 2, a allergen. Blo t 2 was produced as a recombinant protein in . Its allergenic activity was tested in humans by skin prick test and basophil activation assays, and in mice, by passive cutaneous anaphylaxis and a model of allergic airway inflammation. Sensitization rate to Blo t 2 (54.3%) was similar to that found to Blo t 21 (57.2%) and higher than to Der p 2 (37.5%). Most Blo t 2-sensitized patients showed a low intensity response (99.5%). Blo t 2 elicited CD203c upregulation and allergen induced skin inflammation. Additionally, immunized animals produced anti-Blo t 2 IgE antibodies and passive transfer of their serum to non-immunized animals induced skin inflammation after allergen exposure. Immunized animals developed bronchial hyperreactivity and a strong inflammatory lung reaction (eosinophils and neutrophils). These results confirm the allergenic activity of Blo t 2 and supports its clinical relevance.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053487 | PMC |
| http://dx.doi.org/10.3390/ijms24065543 | DOI Listing |
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