AI Article Synopsis
- Peripheral mononuclear blood cells (PBMCs) are commonly used for studying the immune response and identifying specific T-cells, but challenges arise from limited patient samples and the low frequency of antigen-specific T-cells.
- A new platform was developed using HLA-matched immortalized B cells that are modified to enhance the expression of viral or tumor antigens, improving the expansion of antigen-specific T cells from low-frequency samples.
- This innovative method is more efficient, cost-effective, and provides a reliable resource for detecting and expanding antigen-specific T cells, making it a superior alternative to traditional approaches using synthesized peptides.
Article Abstract
Peripheral mononuclear blood cells (PBMCs) are the most widely used study materials for immunomonitoring and antigen-specific T-cell identification. However, limited patient PBMCs and low-frequency antigen-specific T cells remain as significant technical challenges. To address these limitations, we established a novel platform comprised of optimized HLA-matched immortalized B cells transfected with mRNA of a prototype viral or tumor antigen conjugated to MHC class-I trafficking domain protein (MITD) to increase the efficiency of epitope expression in antigen-presenting cells (APCs) essential to expanding antigen-specific T cells. When applied to CMV as a model, the IBMAM platform could successfully expand CMV-specific T cells from low-frequency CMV PBMCs from seropositive donors. Additionally, this platform can be applied to the validation of antigen specific TCRs. Together, compared to using APCs with synthesized peptides, this platform is an unlimited, highly efficient, and cost-effective resource in detecting and expanding antigen-specific T cells and validating antigen-specific TCRs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10045729 | PMC |
| http://dx.doi.org/10.3390/biomedicines11030796 | DOI Listing |
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