Cancer is one of the main causes of human mortality worldwide. Despite the advances in the diagnostics, surgery, radiotherapy, and chemotherapy, the search for more effective treatment regimens and drug combinations are relevant. This work aimed to assess the radiomodifying effect and molecular mechanism of action of fucoidan from the brown alga (ScF) and product of its autohydrolysis (ScF_AH) in combination with pacificusoside D from the starfish (SpD) on the model of viability and invasion of three-dimension (3D) human melanoma cells SK-MEL-2. The cytotoxicity of ScF (IC JB6 Cl41 > 800 µg/mL; IC SK-MEL-2 = 685.7 µg/mL), ScF_AH (IC JB6 Cl41/SK-MEL-2 > 800 µg/mL), SpD (IC JB6 Cl41 = 22 µM; IC SK-MEL-2 = 5.5 µM), and X-ray (ID JB6 Cl41 = 11.7 Gy; ID SK-MEL-2 = 6.7 Gy) was determined using MTS assay. The efficiency of two-component treatment of 3D SK-MEL-2 cells was revealed for ScF in combination with SpD or X-ray but not for the combination of fucoidan derivative ScF_AH with SpD or X-ray. The pre-treatment of spheroids with ScF, followed by cell irradiation with X-ray and treatment with SpD (three-component treatment) at low non-toxic concentrations, led to significant inhibition of the spheroids' viability and invasion and appeared to be the most effective therapeutic scheme for SK-MEL-2 cells. The molecular mechanism of radiomodifying effect of ScF with SpD was associated with the activation of the initiator and effector caspases, which in turn caused the DNA degradation in SK-MEL-2 cells as determined by the Western blotting and DNA comet assays. Thus, the combination of fucoidan from brown algae and triterpene glycoside from starfish with radiotherapy might contribute to the development of highly effective method for melanoma therapy.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046073PMC
http://dx.doi.org/10.3390/biom13030419DOI Listing

Publication Analysis

Top Keywords

fucoidan brown
12
jb6 cl41
12
sk-mel-2 cells
12
brown alga
8
pacificusoside starfish
8
melanoma cells
8
molecular mechanism
8
viability invasion
8
800 µg/ml
8
spd x-ray
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!