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Coupling Environmental Whole Mixture Toxicity Screening with Unbiased RNA-Seq Reveals Site-Specific Biological Responses in Zebrafish. | LitMetric

AI Article Synopsis

  • Passive sampling devices combined with zebrafish toxicity tests effectively detect harmful non-polar organic mixtures in environmental sites.
  • In a study of two locations in Portland Harbor, higher toxicity was noted at river mile 6.5W, linked to specific malformations in zebrafish development.
  • RNA sequencing revealed significant gene expression changes due to exposure, suggesting that while some effects stem from known chemicals, other unidentified contaminants may also contribute to toxicity.

Article Abstract

Passive sampling device (PSD) extracts paired with developmental toxicity assays in (zebrafish) are excellent sensors for whole mixture toxicity associated with the bioavailable non-polar organics at environmental sites. We expand this concept by incorporating RNA-Seq in 48-h post fertilization zebrafish statically exposed to PSD extracts from two Portland Harbor Superfund Site locations: river mile 6.5W (RM 6.5W) and river mile 7W (RM 7W). RM 6.5W contained higher concentrations of polycyclic aromatic hydrocarbons (PAHs), but the diagnostic ratios of both extracts indicated similar PAH sourcing and composition. Developmental screens determined RM 6.5W to be more toxic with the most sensitive endpoint being a "wavy" notochord malformation. Differential gene expression from exposure to both extracts was largely parallel, although more pronounced for RM 6.5W. When compared to the gene expression associated with individual chemical exposures, PSD extracts produced some gene signatures parallel to PAHs but were more closely matched by oxygenated-PAHs. Additionally, differential expression, reminiscent of the wavy notochord phenotype, was not accounted for by either class of chemical, indicating the potential of other contaminants driving mixture toxicity. These techniques offer a compelling method for non-targeted hazard characterization of whole mixtures in an in vivo vertebrate system without requiring complete chemical characterization.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10053777PMC
http://dx.doi.org/10.3390/toxics11030201DOI Listing

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