• Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity. • The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained. • Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated. • Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039704 | PMC |
| http://dx.doi.org/10.1016/j.virs.2023.03.006 | DOI Listing |
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