Objectives: The occurrence of extended-spectrum ß-lactamase (ESBL)-producing Enterobacterales in broilers represents a risk to public health because of the possibility of transmission of ESBL producers and/or bla genes via the food chain or within settings where human-animal interfaces exist.
Methods: This study assessed the occurrence of ESBL producers among faecal samples of broilers at slaughter. Isolates were characterised by multilocus sequence typing, antimicrobial susceptibility testing, and whole-genome sequencing.
Results: The flock prevalence, determined by sampling crates of 100 poultry flocks, was 21%. The predominant bla gene was bla, identified in 92% of the isolates. A variety of Escherichia coli and Klebsiella pneumoniae sequence types (STs) were identified, including extraintestinal pathogenic E. coli ST38, avian pathogenic E. coli ST10, ST93, ST117, and ST155, and nosocomial outbreak clone K. pneumoniae ST20. Whole-genome sequencing was used to characterise a subset of 15 isolates, including 6 E. coli, 4 K. pneumoniae, 1 Klebsiella grimontii, 1 Klebsiella michiganensis, 1 Klebsiella variicola, and 1 Atlantibacter subterranea. Fourteen isolates carried identical or closely related 46338-54929 bp IncX3 plasmids encoding bla and qnrS1. One E. coli isolate carried a 46338 bp IncX3 plasmid, which was integrated chromosomally into ydbD.
Conclusions: The bla gene has replaced the previously predominant bla in ESBL-producing Enterobacterales from broilers in Switzerland. Broilers may play a role in the dissemination of bla and qnrS1 associated with epidemic IncX3 plasmids, representing a risk to human and animal health.
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http://dx.doi.org/10.1016/j.jgar.2023.03.008 | DOI Listing |
Foodborne Pathog Dis
January 2025
Center for Disease Control and Prevention of Sichuan Province, Chengdu, China.
In recent years, infection is a major global public health concern, particularly in food safety. This study analyzed the genomes of 102 strains isolated between 2016 and 2023 from food, foodborne disease patients, and food poisoning incidents, focusing on their molecular characteristics, antibiotic resistance genes (ARGs), and virulence genes. serovar Enteritidis (37.
View Article and Find Full Text PDFFront Pediatr
January 2025
Department of Hematology, Aerospace Center Hospital, Beijing, China.
Introduction: Hypervirulent carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) poses an increasing public health risk due to its high treatment difficulty and associated mortality, especially in bone marrow transplant (BMT) patients. The emergence of strains with multiple resistance mechanisms further complicates the management of these infections.
Methods: We isolated and characterized a novel ST11-KL64 hv-CRKP strain from a pediatric bone marrow transplantation patient.
J Glob Antimicrob Resist
December 2024
Department of Clinical Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, China. Electronic address:
Braz J Microbiol
December 2024
Department of Clinical Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, No. 365, East renmin Rd, Zhejiang, Jinhua, People's Republic of China.
Ann Clin Microbiol Antimicrob
September 2024
Department of Clinical Laboratory, Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, 310022, Zhejiang, China.
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