AI Article Synopsis
- Hirame novirhabdovirus (HIRRV) is a major viral threat to Japanese flounder, leading to this study focused on creating monoclonal antibodies (mAbs) for detection.
- Seven mAbs were produced, with three targeting the nucleoprotein and four targeting the matrix protein of HIRRV, showcasing specificity without cross-reactivity to other fish viruses.
- The mAbs, primarily of the IgG1 class, promise to enhance immunodiagnosis methods for HIRRV infections in fish.
Article Abstract
Hirame novirhabdovirus (HIRRV) is a significant viral pathogen of Japanese flounder (). In this study, seven monoclonal antibodies (mAbs) against HIRRV (isolate CA-9703) were produced and characterized. Three mAbs (1B3, 5G6, and 36D3) were able to recognize nucleoprotein (N) (42 kDa) and four mAbs (11-2D9, 15-1G9, 17F11, and 24-1C6) recognized matrix (M) protein (24 kDa) of HIRRV. Western blot, Enzyme-linked immunosorbent assay, and indirect fluorescent antibody technique (IFAT) results indicated that the developed mAbs were specific to HIRRV without any cross-reactivity against other different fish viruses and epithelioma papulosum cyprini cells. All the mAbs comprised IgG1 heavy chain and κ light chain except 5G6, which has a heavy chain of IgG2a class. These mAbs can be very useful in development of immunodiagnosis of HIRRV infection.
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| http://dx.doi.org/10.1089/mab.2022.0040 | DOI Listing |
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