Purpose: To discuss the effects of dragon's blood extract, a Chinese herb, on periodontal tissue repair and toll like receptor 4/nuclear transcription factor kappa B(TLR4/NF-κB) in gingivitis rats.
Methods: Sixty rats were randomly divided into control group, gingivitis group and low, medium and high dose groups of dragon's blood extract, with 10 rats in each group. Except the control group, the gingivitis rat model was established by silk thread ligation in other groups. The model was established successfully. The low, medium and high dose groups rats were given 150, 300 and 600 mg·kg·d dragon's blood extract successively by gavage (once a day for 4 weeks). Rats in the model group and the control group were given the same amount of normal saline by gavage at the same time. After the rats were sacrificed under anesthesia, the jaw tissue of the left maxillary second molar was stained with methylene blue to observe and measure the loss of alveolar bone (ABL), H-E staining was used to observe the pathological changes of periodontal tissue (jaw tissue). The levels of IL-17 and IL-4 in periodontal tissue (jaw tissue) of rats in each group were detected by enzyme-linked immunosorbent assay (ELISA). Bone morphogenetic protein-2(BMP-2), TLR4 and NF-κB p65 protein level in rat periodontal tissue were detected by Western blot. SPSS 19.0 software package was used to analyze the data.
Results: Compared with the control group, IL-17, IL-4, TLR4 and NF-κB p65 protein and ABL in jaw tissue of model group were significantly increased (P＜0.05), and the level of BMP-2 protein in jaw tissue significantly decreased (P＜0.05). Compared with the model group, IL-17, IL-4, TLR4 and NF-κB p65 protein and ABL in jaw tissue of rats in low, medium and high dose groups of dragon's blood extract were significantly increased, and the level of BMP-2 protein significantly decreased(P＜0.05).
Conclusions: Dragon's blood extract can inhibit TLR4/NF- κB. Activation of B pathway inhibits inflammatory response and promotes periodontal tissue repair in gingivitis rats.
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