Remodeling of the gut microbiome by alleviates the development of acute myocardial infarction.

Introduction: The gut microbial community, which can be disturbed or repaired by changes in the internal environment, contributes to the development of acute myocardial infarction (AMI). Gut probiotics play a role in microbiome remodeling and nutritional intervention post-AMI. A newly isolated strain EU03 has shown potential as a probiotic. Here, we investigated the cardioprotective function and mechanism of through gut microbiome remodeling in AMI rats.

Methods: A rat model of left anterior descending coronary artery ligation (LAD)-mediated AMI was assessed with echocardiography, histology, and serum cardiac biomarkers to evaluate the beneficial effects of . The immunofluorescence analysis was utilized to visualize the intestinal barrier changes. Antibiotic administration model was used for assessing the gut commensals' function in the improvement of cardiac function post-AMI. The underlying beneficial mechanism through enrichment was further investigated by metagenomics and metabolomics analysis.

Results: A 28-day treatment with protected cardiac function, delayed cardiac pathology, suppressed myocardial injury cytokines, and improved gut barrier integrity. The microbiome composition was reprogrammed by enhancing the abundance of . Microbiome dysbiosis by antibiotics abrogated the improvement of cardiac function post-AMI by . enrichment caused remodeling of gut microbiome by increasing the abundance of , , and decreasing , UCG-014, which were correlated with cardiac traits and serum metabolic biomarkers 16,16-dimethyl-PGA2, and Lithocholate 3-O-glucuronide.

Conclusion: These findings reveal that gut microbiome remodeling by ameliorates the cardiac function post-AMI and might advance microbiome-targeted nutritional intervention.Graphical Abstract.