AI Article Synopsis
- Efficiently isolating antigen-specific B cells can speed up the discovery of therapeutic monoclonal antibodies (mAbs) and improve vaccine development.
- Traditional methods for mAb discovery are time-consuming and expensive, but new techniques in single-cell genomics enable the processing of thousands of cells at once.
- The introduced method combines antigen barcoding and computational tools to analyze large numbers of B cells, successfully recovering thousands of mAbs, including rare precursors for key HIV-neutralizing antibodies.
Article Abstract
The ability to efficiently isolate antigen-specific B cells in high throughput will greatly accelerate the discovery of therapeutic monoclonal antibodies (mAbs) and catalyze rational vaccine development. Traditional mAb discovery is a costly and labor-intensive process, although recent advances in single-cell genomics using emulsion microfluidics allow simultaneous processing of thousands of individual cells. Here we present a streamlined method for isolation and analysis of large numbers of antigen-specific B cells, including next generation antigen barcoding and an integrated computational framework for B cell multi-omics. We demonstrate the power of this approach by recovering thousands of antigen-specific mAbs, including the efficient isolation of extremely rare precursors of VRC01-class and IOMA-class broadly neutralizing HIV mAbs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036767 | PMC |
| http://dx.doi.org/10.3389/fcimb.2022.962945 | DOI Listing |
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