AI Article Synopsis
- Metastasis is a major cause of death in breast cancer, and circular RNAs, particularly circRRM2, are implicated in the disease's progression, although their precise roles are still being investigated.
- Research involved analyzing circRNA expression through microArray and validating findings with clinical samples, revealing that circRRM2 is significantly higher in tumor tissues and linked to advanced cancer stages.
- Functional experiments demonstrated that circRRM2 acts as an oncogene promoting cell migration and invasion, creating a positive feedback loop with IGF2BP1 and MYC, which may be a critical pathway in breast cancer metastasis.
Article Abstract
Background: Metastasis is the leading cause of mortality in patients with breast cancer (BC). Studies demonstrate that circular RNAs (circRNAs) were involved in BC progression, while the molecular mechanisms remain largely unclear.
Methods: The microArray circRNA profiles were used to explore the differential expression circRNAs in BC and paracancerous normal tissues, and the quantitative reverse transcription-polymerase chain reaction was used to validate their expression level in clinical samples and cell lines. Nuclear/cytosolic fractionation and fluorescence in situ hybridization (FISH) assays were performed to examine circRRM2 (hsa_circ_0052582) subcellular location. The scratch wound healing and transwell assays were conducted to evaluate the impact of circRRM2 on BC cell migration and invasion. We predicted miRNAs that might bind with cricRRM2 and the downstream target genes using bioinformatics analysis and explored their expression levels and prognostic value in BC. FISH, RNA immunoprecipitation, Co-immunoprecipitation, Western blot, and rescue experiments were implemented to figure out circRRM2 function and underlying mechanisms in BC.
Results: The present study revealed several aberrant circRNAs in BC tissues and observed that circRRM2 was upregulated in tumor tissues of 40 patients with BC. High circRRM2 was significantly associated with advanced N stage in patients with BC. Gain- and loss- of function experiments revealed that circRRM2 promoted the migration and invasion of cells and functioned as an oncogene in BC. Mechanism studies showed that circRRM2 competed with miR-31-5p/miR-27b-3p to upregulate the IGF2BP1 expression. Furthermore, IGF2BP1 upregulated the circRRM2 level via interacting with MYC, which functioned as the transcriptional factor of circRRM2. Thus, the positive feedback loop that was composed of circRRM2/IGF2BP1/MYC was identified.
Conclusion: This study confirms that upregulated circRRM2 functions an oncogenic role in BC metastasis. The positive feedback loop of circRRM2/IGF2BP1/MYC enforces the circRRM2 expression, which might offer a potential target for BC treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10039515 | PMC |
| http://dx.doi.org/10.1186/s12935-023-02895-w | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Circular RNA RRM2 alleviates metabolic dysfunction-associated steatotic liver disease by targeting miR-142-5p to increase NRG1 expression.
Am J Physiol Gastrointest Liver Physiol
October 2024
Department of Preventive Medicine, Shantou University Medical College, Shantou, People's Republic of China.
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a prevalent chronic liver condition worldwide, demanding further investigation into its pathogenesis. Circular RNAs (circRNAs) are emerging as pivotal regulators in MASLD processes, yet their pathological implications in MASLD remain poorly understood. This study focused on elucidating the role of circular RNA ribonucleotide reductase subunit M2 (circRRM2) in MASLD progression.
View Article and Find Full Text PDFA novel feedback regulated loop of circRRM2-IGF2BP1-MYC promotes breast cancer metastasis.
Cancer Cell Int
March 2023
Department of Breast Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Article Synopsis
- Metastasis is a major cause of death in breast cancer, and circular RNAs, particularly circRRM2, are implicated in the disease's progression, although their precise roles are still being investigated.
- Research involved analyzing circRNA expression through microArray and validating findings with clinical samples, revealing that circRRM2 is significantly higher in tumor tissues and linked to advanced cancer stages.
- Functional experiments demonstrated that circRRM2 acts as an oncogene promoting cell migration and invasion, creating a positive feedback loop with IGF2BP1 and MYC, which may be a critical pathway in breast cancer metastasis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!