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CRISPR/Cas12a regulated preassembled bulb-shaped G-quadruplex signal unit for FL/CM dual-mode ultrasensitive detection of miRNA-155.
Talanta
December 2024
Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China; Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, PR China. Electronic address:
High sensitivity and specificity in microRNA detection are of great significance for early cancer screening. This study employed a pre-assembled bulb-shaped G-quadruplex signal unit (G4MB) as a novel and efficient label-free probe. The products amplified by the miRNA-155-targeted exponential amplification reaction (EXPAR) activated the trans-cleavage activity of CRISPR/Cas12a, disrupting the G4MB structure to achieve dual-channel fluorescence/colorimetric (FL/CM) inverse signal output.
View Article and Find Full Text PDFConstruction of a N-CDs/AuNCs@ZIF-8-assisted ratiometric fluorescent nanosensor for glyphosate detection in edible and medicinal malt.
Food Chem X
December 2024
State Key Laboratory of Southwestern Chinese Medicine Resources, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Glyphosate (Gly) is a widely-used herbicide in food production, while, the residue of which due to the long-term and excessive overspray poses serious threats to public health. The development of reliable methods for its sensitive detection is of great urgency. In this study, a novel ratiometric fluorescent nanosensor by encapsulating N-doped carbon dots (N-CDs) and gold nanoclusters (AuNCs) in zeolitic imidazole framework-8 (ZIF-8) as the dual-emissive fluorescence probes at 410 and 650 nm, respectively, was constructed for Gly detection.
View Article and Find Full Text PDFHelicoid Grating-Coupled Surface Plasmon Resonance Sensor.
Nano Lett
December 2024
Department of Materials Science and Engineering, Seoul National University, Seoul 08826, Republic of Korea.
Ultrasensitive, rapid, and reliable biomolecular sensing is essential for biomedical diagnostics, requiring real-time monitoring and detection of trace samples. Optical sensing, particularly plasmonic biosensing, meets these demands through noninvasive, high-sensitivity detection based on the interaction between light and molecules. Here, we present novel plasmonic metamaterial-based sensing strategy, utilizing the circular dichroism (CD) response of grating-coupled surface plasmon resonance (SPR) from chiral nanoparticle grating structure (i.
View Article and Find Full Text PDFCorrection: Hu et al. Ultrasensitive Silicon Nanowire Biosensor with Modulated Threshold Voltages and Ultra-Small Diameter for Early Kidney Failure Biomarker Cystatin C. 2023, , 645.
Biosensors (Basel)
September 2024
Advanced Integrated Circuits R&D Center, Institute of Microelectronic of the Chinese Academy of Sciences, Beijing 100029, China.
Article Synopsis
- The original publication discusses the main themes and ideas presented in the work.
- It highlights the significance of the research and its impact on the field.
- Additionally, it summarizes the key findings and conclusions drawn by the authors.
Next-generation CRISPR/Cas-based ultrasensitive diagnostic tools: current progress and prospects.
RSC Adv
October 2024
Department of Pharmacy, Birla Institute of Technology and Science, Pilani Vidya Vihar Pilani 333031 Rajasthan India +91 01596 515 835 +91 9660 456 009.
Article Synopsis
- CRISPR/Cas acts like molecular scissors for precise gene editing using mechanisms like non-homologous end joining (NHEJ) or homology-directed repair (HDR), with various Cas effectors available for different applications.
- SpCas9 is the most studied version, recognized as a breakthrough for correcting genetic mutations, while newer variants like Cas12 and Cas13 are noted for their specific abilities to recognize and cleave target nucleic acids.
- The combination of CRISPR technology, diagnostics, and nanotechnology has led to the development of advanced biosensing systems capable of providing quantitative fluorescent signals for diagnostic purposes.
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