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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034874 | PMC |
| http://dx.doi.org/10.1007/s10142-023-01024-0 | DOI Listing |
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Nucleic Acid Detection through RNA-Guided Protease Activity in Type III-E CRISPR-Cas Systems.
Chembiochem
November 2023
The Province and Ministry Co-sponsored Collaborative, Innovation Center for Medical Epigenetics, Key Laboratory of Immune Microenvironment and, Disease (Ministry of Education), Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin, 300070, P. R. China.
RNA-guided protease activity was recently discovered in the type III-E CRISPR-Cas systems (Craspase), providing a novel platform for engineering a protein probe instead of the commonly used nucleic acid probe in nucleic acid detection assays. Here, by adapting a fluorescence readout technique using the affinity- and fluorescent protein dual-tagged Csx30 protein substrate, we have established an assay monitoring Csx30 cleavage by target ssRNA-activated Craspase. Four Craspase-based nucleic acid detection systems for genes from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), norovirus, and the influenza virus (IFV) were reconstituted with demonstrated specificity.
View Article and Find Full Text PDFCraspase: A novel CRISPR/Cas dual gene editor.
Funct Integr Genomics
March 2023
Department of Biology, East Carolina University, Greenville, NC, 27858, USA.
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