AI Article Synopsis
- The study investigates the role of circular RNA_0002715 (circ_0002715) in the progression of osteoarthritis (OA).
- Silencing circ_0002715 reduces inflammation, cell death, and protein degradation in OA cell models by sponging microRNA-127-5p, which then targets Latexin (LXN).
- The findings suggest that circ_0002715 could be a new therapeutic target for OA by regulating the miR-127-5p/LXN pathway to influence chondrocyte injury.
Article Abstract
Background: Our study aims to investigate the role and mechanism of circular RNA_0002715 (circ_0002715) in osteoarthritis (OA) progression.
Methods: IL-1β-induced CHON-001 cells were used to mimic OA cell model. Circ_0002715, microRNA (miR)-127-5p and Latexin (LXN) expression was detected by quantitative real-time PCR. Cell functions were determined by MTT assay, flow cytometry and ELISA assay. Protein expression was examined by western blot.
Results: Circ_0002715 was highly expressed in OA cartilage tissues. Circ_0002715 silencing inhibited inflammation, apoptosis, and ECM degradation in IL-1β-interfered CHON-001 cells. Circ_0002715 could sponge miR-127-5p, and miR-127-5p could target LXN. The effect of circ_0002715 down-regulation on chondrocyte injury was partially restored by miR-127-5p inhibitor. MiR-127-5p could suppress chondrocyte injury by inhibiting LXN expression.
Conclusion: Circ_0002715 might be a new therapeutic target for OA, which regulated miR-127-5p/LXN axis to promote IL-1β-induced chondrocyte injury.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031964 | PMC |
| http://dx.doi.org/10.1186/s13018-023-03638-3 | DOI Listing |
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