Spheroplasts in the urinary sediment.

Kidney Int

Clinical and Research Laboratory on Urinary Sediment, SC di Nefrologica, Dialisi, e Trapianto di Rene, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milano, Italia.

Published: April 2023

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http://dx.doi.org/10.1016/j.kint.2022.12.009DOI Listing

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Spheroplasts in the urinary sediment.

Kidney Int

April 2023

Clinical and Research Laboratory on Urinary Sediment, SC di Nefrologica, Dialisi, e Trapianto di Rene, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milano, Italia.

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Spheroplasts, poorly known but clinically relevant particles of urinary sediment.

Clin Chim Acta

April 2021

Laboratory of Clinical Chemistry, Desio Hospital, ASST-Monza and Department of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.

Background: In two patients under treatment with various antibiotics, spheroplasts were detected with an automated urine sediment analyzer.

Methods: Urinalysis was performed by an AutionMAX AX 4030-sediMAX platform.

Results: Spheroplasts can be easily misclassified as yeasts or erythrocytes, but when automated urine sediment analyzers are used by well-trained, and experienced operators they can be correctly identified and classified.

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A 60 year-old man was submitted to kidney transplantation in 2013 due to chronic renal insufficiency caused by hypertension. He had recurrent episodes of urinary tract infection and came to the hospital due to a 4 day-long fever, abdominal pain, burning urination and nausea. Routine urinalysis revealed a picture of infection (> 50 leucocytes/high power field associated to massive bacteriuria).

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The bacteriocin PsVP-10 is a 2.6 Kda peptide which was isolated and purified from Pseudomonas sp. This bacteriocin possesses lethal activity over Enterococcus faecalis, Salmonella typhimurium and Shigella flexneri.

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Cystic forms (also called spheroplasts or starvation forms) and their ability to reconvert into normal motile spirochetes have already been demonstrated in the Borrelia burgdorferi sensu lato complex. The aim of this study was to determine whether motile B. garinii could develop from cystic forms, not only in vitro but also in vivo, in cyst-inoculated mice.

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