Background: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs.
Methods: hUCMSCs were isolated from Wharton's jelly. Subsequently, hUCMSCs were exposed to cold storage (4 °C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated.
Results: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2-related factor 2 signaling.
Conclusion: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219924 | PMC |
| http://dx.doi.org/10.1007/s13770-023-00531-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Effectiveness of Prolonged Hypothermic Preservation of Isolated Rat Hearts Using Oxygen, Medical Nitrous Oxide and Carbon Monoxide Gas Mixtures.
Arch Biochem Biophys
January 2025
Institute of Cell Biophysics RAS - a separate subdivision of Federal Research Centre "Pushchino Scientific Centre for Biological Research RAS", Institutskaya St., 3, 142290, Russia, Moscow Region, Pushchino. Electronic address:
The possibility of using an oxygen-nitrous oxide mixture for prolonged hypothermic preservation of rat heart for 24 hours was investigated. A comparative analysis of restoration of functional activity of hearts in the groups of 24-hour preservation at +4°C with different gases (O, N) and gas mixtures (CO+O, NO+O, N+O, NO+N) was carried out. It was shown that the presence of oxygen in the gas mixture was the key factor for heart preservation.
View Article and Find Full Text PDFRetrospective Clinical Outcomes of Keratoplasty Using Human Donor Corneas Preserved in Eusol-C Hypothermic Storage Medium.
J Clin Med
December 2024
Ophthalmology Unit-Eye Bank of Rome, San Giovanni Addolorata Hospital, 00184 Rome, Italy.
To evaluate the clinical outcomes of cornea transplantation (penetrating keratoplasty, Descemet membrane endothelial keratoplasty, Descemet stripping automated endothelial keratoplasty, and deep anterior lamellar keratoplasty) using donor corneas stored in Eusol-C hypothermic storage medium compared to corneas stored in organ-culture. : The clinical outcomes of 92 patients who underwent corneal transplantation with human donor corneas stored in Eusol-C medium at 2-8 °C were retrospectively evaluated. The control group consisted of 169 patients who received corneas organ-cultured at 31 °C.
View Article and Find Full Text PDFThe Evolution of Kidney Graft Preservation Through the Years.
Life (Basel)
December 2024
Department of Urology, Clínica Universidad de Navarra, 31008 Pamplona, Spain.
Chronic kidney disease (CKD) is a prevalent disease affecting almost 10% of the world's population, with many cases progressing to end-stage kidney disease (ESKD). Kidney transplantation (KT) is the gold-standard treatment for ESKD. Due to growing KT waitlists, the deceased kidney donor (DKDs) criteria have expanded to increase the number of available kidney grafts.
View Article and Find Full Text PDFXenotransplantation (XTx) is an increasingly realistic solution to the organ shortage. Clinical XTx may require off-site procurement in a designated pathogen free (DPF) facility necessitating a period of cold ischemic time during transportation. This study evaluates the impact of different kidney preservation strategies on early graft function in pig-to-baboon XTx in a series of eight cases of pig-to-baboon xenotransplantation performed after five hours of cold ischemic time and compares these results to six cases of pig-to-baboon xenotransplantation performed with minimal ischemic time.
View Article and Find Full Text PDFDanger Biomarkers in Perfusates From Fatty Liver Grafts Subjected to Cold Storage Preservation in Different Preservation Solutions.
Transplant Proc
January 2025
Experimental Pathology Department, Institut d'Investigacions Biomèdiques de Barcelona (IIBB)-CSIC, Barcelona, Spain; Steatohepatitis and Liver Transplant, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), CIBER ehd, Barcelona, Spain.
Static cold storage remains the traditional standard for liver graft preservation prior to transplantation in both clinical and experimental settings. The use of polyethylene glycol 35 solutions, such as Institut Georges Lopez-2 (IGL2) preservation solution, for protecting against mitochondrial damage during cold static preservation necessitates combination with hypothermic oxygenated perfusion to enhance liver graft performance. This study presents a preliminary comparative evaluation of "danger signals" indicating hepatocellular injury (transaminases, lactate content), mitochondrial damage (glutamate dehydrogenase release), and cytokine release in liver perfusates from suboptimal grafts (fatty livers) subjected to 24-hour cold storage.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!