Two previously undescribed flavonoid thioglucosides lepidiumflavonosides A and B () and two known megastigmane compounds (7,9)-9-hydroxy-5,7-megastigmadien-4-one 9-O--D-glucopyranoside () and (9)-4-oxo--inol -D-glucopyranoside () were isolated from the water extract of the seeds of Willd. The structural elucidation of isolated compounds was unambiguously determined based on extensive 1D and 2D NMR spectroscopic analyses. All compounds were evaluated for their estrogen-like effects on MCF-7 cells in . The results showed that compounds significantly promoted the proliferation of MCF-7 cells, and the proliferation was antagonized by the specific ER antagonist ICI182,780, suggesting that compounds might have the estrogen-like effect in potentially.
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