The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present the urobiome of healthy infant males from samples collected by transurethral catheterization. Using a combination of extended culture and amplicon sequencing, we identify several common bacterial genera that can be further investigated for their effects on urinary health across the lifespan. Many genera were shared between all samples suggesting a consistent urobiome composition among this cohort. We note that, for this cohort, early life exposures including mode of birth (vaginal vs. Caesarean section), or prior antibiotic exposure did not influence urobiome composition. In addition, we report the isolation of culturable bacteria from the bladders of these infant males, including , a bacterial species that has been associated with urinary tract infection in older male adults. Herein, we isolate and sequence 9 distinct strains of enhancing the genomic knowledge surrounding this species and opening avenues for delineating the microbiology of this urobiome constituent. Furthermore, we present a framework for using the combination of culture-dependent and sequencing methodologies for uncovering mechanisms in the urobiome.
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http://dx.doi.org/10.21203/rs.3.rs-2618137/v1 | DOI Listing |
Diagnostics (Basel)
January 2025
Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi 110029, India.
: The complex interaction between the gut and urinary microbiota underscores the importance of understanding microbial dysbiosis in pediatric urinary tract infection (UTI). However, the literature on the gut-urinary axis in pediatric UTIs is limited. This systematic review aims to summarize the current literature on the roles of gut and urinary dysbiosis in pediatric UTIs.
View Article and Find Full Text PDFTranspl Immunol
January 2025
Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada. Electronic address:
Characteristic alterations in the urinary microbiome, or urobiome, are associated with renal transplant pathology. To date, there has been no direct study of the urobiome during acute allograft rejection. The goal of this study was to determine if unique urobiome alterations are present during acute rejection in renal transplant recipients.
View Article and Find Full Text PDFPathogens
November 2024
Internal Medicine Department, San Carlo di Nancy Hospital, GVM Care & Research, 00165 Rome, Italy.
Urinary tract infections (UTIs) are one of the most common bacterial infections, affecting more than 150 million people each year in the world. UTIs have grown exponentially in the last few years. They represent a major load for both individuals and society.
View Article and Find Full Text PDFEur Rev Med Pharmacol Sci
December 2024
ASST Sette Laghi, Circolo e Fondazione Macchi Hospital, University of Insubria, Varese, Italy.
Benign prostatic hyperplasia (BPH) is highly prevalent and associated with a significant impact on individuals' well-being. Initial management involves various medications, but their benefits can be limited by side effects, particularly concerning young people. In this context, novel and better-tolerated therapeutic strategies have been proposed, thus including the modulation of the gut microbiome through probiotic ingestion.
View Article and Find Full Text PDFNat Commun
December 2024
Department of Cardiovascular and Metabolic Sciences, Cleveland Clinic, Cleveland, OH, USA.
Clinical studies of the urinary tract microbiome, termed urobiome, suggest a direct, antibiotic-dependent, impact of the urobiome on kidney physiology. However, evidence for kidney bacteria comes from indirect sources or infected tissue. Further, it is unclear how antibiotics impact kidney bacteria.
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