AI Article Synopsis
- Trehalase is a crucial enzyme that breaks down trehalose, a type of sugar, in various organisms.
- The study introduces new fluorometric nanoprobes using trehalose-functionalized quantum dots (QDs) to detect trehalase activity.
- When trehalose is cleaved by trehalase, the QDs form aggregates that can be measured, allowing for specific and efficient detection of the enzyme based on its concentration.
Article Abstract
Trehalase is an important enzyme in the metabolic cascades of many organisms, catalysing the hydrolysis of the disaccharide trehalose. Herein we describe the first examples of fluorometric nanoprobes for detection of trehalase, based on trehalose-functionalised quantum dots (QDs). QDs cross-linked with trehalose form aggregates, which are released upon enzymatic cleavage of the trehalose glycosidic bond proportionally to the enzyme concentration, offering a unique and efficient approach for specific sensing of this biologically important enzyme.
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| http://dx.doi.org/10.1039/d3ob00368j | DOI Listing |
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