Association of Different Combinations of rs671, rs429358, rs7412 Polymorphisms with Hypertension in Middle-Aged and Elderly People: A Case-Control Study.

Background: Hypertensive patients have a younger trend, and studies on the role of genetic factors in hypertension susceptibility have been inconsistent. Aldehyde dehydrogenases 2 (ALDH2) and apolipoprotein E (APOE) are involved in the pathophysiological processes of hypertension. To investigate the relationship of and polymorphisms with hypertension in middle-aged (30-59 years old) and elderly (≥60 years old) persons.

Methods: Two thousand six hundred and ten hypertensive patients and 1921 controls were included (between 30 and 100 years old). The genotypes of common polymorphisms in and genes ( rs429358, rs7412, and rs671) of the subjects were analyzed by polymerase-chain reaction (PCR)-microarray. Statistical analyses (Student's -test, Mann-Whitney -test, test, and logistic regression analysis) were performed with SPSS v21.0.

Results: There were 4531 participants (66.60 ± 12.10 years old) in this study, including 3057 (67.5%) males and 1474 (32.5%) females. There were no significant differences in distributions of 2 rs671, rs429358/rs7412 genotypes and alleles between hypertensive patients and controls. Persons with rs671 G/A or A/A genotype were less likely to have hypertension (G/A+A/A vs G/G: gender-, age-, smoking-, and drinking-adjusted OR 0.885, 95% CI 0.785-0.997, =0.045), while rs671 A/A+ rs429358 or rs7412 wild-type genotype may decrease the risk of hypertension. In middle-aged group, rs671 G/A+ rs429358 T/C carriers (adjusted OR 0.547, 95% CI 0.350-0.856, =0.008), and rs671 A/A+ rs7412 C/C genotypes (adjusted OR 0.567, 95% CI 0.361-0.891, =0.014) were less likely to have hypertension. In elderly group, rs7412 T/T carriers were more likely to have hypertension (rs671 T/T vs C/C: adjusted OR 4.755, 95% CI 1.075-21.027, =0.040; rs671 T/T vs C/C or C/T: adjusted OR 4.734, 95% CI 1.071-20.928, =0.040).

Conclusion: Polymorphism-polymorphism interactions of rs671 and rs429358/rs7412 may effect on hypertension susceptibility. Different genotypes comparison shows different roles in middle-aged and elderly people, respectively.