Combination of epigenetic erasing and mechanical cues to generate human epiBlastoids from adult dermal fibroblasts.

J Assist Reprod Genet

Department of Veterinary Medicine and Animal Science, Center for Stem Cell Research, Laboratory of Biomedical Embryology and Tissue Engineering, Università Degli Studi Di Milano, 26900, Lodi, Italy.

Published: May 2023

Purpose: This study is to develop a new protocol that combines the use of epigenetic cues and mechanical stimuli to assemble 3D spherical structures, arbitrarily defined "epiBlastoids," whose phenotype is remarkably similar to natural embryos.

Methods: A 3-step approach is used to generate epiBlastoids. In the first step, adult dermal fibroblasts are converted into trophoblast (TR)-like cells, combining the use of 5-azacytidine, to erase the original phenotype, with an ad hoc induction protocol, to drive cells towards TR lineage. In the second step, epigenetic erasing is applied once again, in combination with mechanosensing-related cues, to generate inner cell mass (ICM)-like organoids. Specifically, erased cells are encapsulated into micro-bioreactors to promote 3D cell rearrangement and boost pluripotency. In the third step, TR-like cells are co-cultured with ICM-like spheroids in the same micro-bioreactors. Subsequently, the newly generated embryoids are transferred to microwells to favor epiBlastoid formation.

Results: Adult dermal fibroblasts are successfully readdressed towards TR lineage. Cells subjected to epigenetic erasing and encapsulated into micro-bioreactors rearrange in 3D ICM-like structures. Co-culture of TR-like cells and ICM-like spheroids into micro-bioreactors and microwells induces the formation of single structures with uniform shape reminiscent in vivo embryos. CDX2 cells localized in the out layer of the spheroids, while OCT4 cells in the inner of the structures. TROP2 cells display YAP nuclear accumulation and actively transcribed for mature TR markers, while TROP2 cells showed YAP cytoplasmic compartmentalization and expressed pluripotency-related genes.

Conclusion: We describe the generation of epiBlastoids that may find useful application in the assisted reproduction field.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10024007PMC
http://dx.doi.org/10.1007/s10815-023-02773-4DOI Listing

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