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is a widespread foodborne pathogen contaminating foods during their production or processing stages. Fresh meat is susceptible to such contamination if it is not properly preserved. Our study was conducted to reveal the level of contamination and prevalence of spp. present in livestock and poultry meat from Gansu province. A total of 1,387 samples were collected from five cities in Gansu Province according to standard sampling procedures, of which 174 samples (12.5%) were positive for species. Among them, 14 isolates of (1.0%), 150 isolates of (10.8%), and ten isolates of (0.7%) were identified by conventional bacteriological and molecular identification methods. All isolates were subjected to serological assays, antimicrobial susceptibility tests, growth curve assays, determination of biofilm-forming capacity, and cluster analysis of the 16S rRNA gene sequences. Four predominant serotypes of were identified, including 1/2a (35.7%, 5/14), 1/2b (14.3%, 2/14), 1/2c (42.9%, 6/14), and 4b (7.1%, 1/14). All isolates were resistant to tetracycline and cefoxitin. Most isolates (63.6%, 14/22) and (40%, 4/10) were resistant to tetracycline. The high biofilm-forming ability was observed among 1/2c and 1/2a serotype isolates. The cluster analysis of the 16S rRNA gene sequences revealed a close genetic relationship between the three species. This study fills the gap in the knowledge of livestock and poultry meat that carry in slaughterhouses and markets in Gansu Province.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10280304 | PMC |
| http://dx.doi.org/10.33073/pjm-2023-002 | DOI Listing |
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