AI Article Synopsis
- In a study on multienzyme cascade reactions, the spacing between enzymes is crucial for optimal activity.
- A Y-shaped DNA scaffold was created on magnetic microspheres to coimmobilize glucose oxidase (GOD) and horseradish peroxidase (HRP), allowing for precise control of their distance.
- The optimal distance between the enzymes for maximum cascade activity was found to be 13.6 nm, which significantly improved performance compared to free enzymes, highlighting the importance of inter-enzyme spacing in enzyme function.
Article Abstract
In multienzymes cascade reaction, the inter-enzyme spacing is supposed to be a factor affecting the cascade activity. Here, a simple and efficient Y-shaped DNA scaffold is assembled using two partially complementary DNA single strands on magnetic microspheres, which is used to coimmobilize glucose oxidase (GOD) and horseradish peroxidase (HRP). As a result, on poly(vinyl acetate) magnetic microspheres (PVAC), GOD/HRP-DNA@PVAC multienzyme system is obtained, which can locate GOD and HRP accurately and control the inter-enzyme distance precisely. The distance between GOD and HRP is regulated by changing the length of DNA strand. It showed that the cascade activity is significantly distance-dependent. Moreover, the inter-enzyme spacing is not the closer the better, and too short distance would generate steric hindrance between enzymes. The cascade activity reached the maximum value of 967 U mg at 13.6 nm, which is 3.5 times higher than that of free enzymes. This is ascribed to the formation of substrate channeling.
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| http://dx.doi.org/10.1002/smll.202301413 | DOI Listing |
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