AI Article Synopsis
- Histamine (HA) plays a vital role in various processes in the central and peripheral nervous systems, and understanding its real-time measurement is crucial for studying its functions.
- A new set of genetically encoded sensors, known as GRAB HA sensors, allows for the precise monitoring of HA release with high photostability, fast response times, and strong specificity.
- Using these sensors, researchers observed patterns of HA release in specific brain areas during different states (like sleep and wakefulness) in mice, highlighting their potential to explore HA dynamics in health and disease.
Article Abstract
Histamine (HA) is a key biogenic monoamine involved in a wide range of physiological and pathological processes in both the central and peripheral nervous systems. Because the ability to directly measure extracellular HA in real time will provide important insights into the functional role of HA in complex circuits under a variety of conditions, we developed a series of genetically encoded G-protein-coupled receptor-activation-based (GRAB) HA (GRAB) sensors with good photostability, sub-second kinetics, nanomolar affinity, and high specificity. Using these GRAB sensors, we measured electrical-stimulation-evoked HA release in acute brain slices with high spatiotemporal resolution. Moreover, we recorded HA release in the preoptic area of the hypothalamus and prefrontal cortex during the sleep-wake cycle in freely moving mice, finding distinct patterns of HA dynamics between these specific brain regions. Thus, GRAB sensors are robust tools for measuring extracellular HA transmission in both physiological and pathological processes.
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| http://dx.doi.org/10.1016/j.neuron.2023.02.024 | DOI Listing |
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