Approximation of circle number π by spread-plating Escherichia coli.

Biochem Mol Biol Educ

Biotechnology Department, Faculty of Life Sciences, Hamburg University of Applied Sciences (HAW Hamburg), Hamburg, Germany.

Published: May 2023

Ideally, microbial spread plating results in randomly distributed colonies on the agar surface. This can be seen as a Monte Carlo simulation and enables probabilistic approximation of circle number π. We perform π approximation in a microbiology undergraduate course to awaken the students' ambition for a good spread plating technique.

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http://dx.doi.org/10.1002/bmb.21723DOI Listing

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