Production of herbal toothpaste: Physical, organoleptic, phyto-compound, and antimicrobial properties.

Objective: To investigate the possibility of producing dental antimicrobial toothpaste from L skin chaff, seed, and pod extracts.

Methods: Ethanolic extracts of the three plant materials were obtained. These were subjected to phytochemical and GC-MS analyses. The different extract combinations were used for the production of various toothpaste. The toothpaste's physical, organoleptic, and antimicrobial properties were determined.

Results: From the phytochemical analysis, has the highest phenolic (1.20 mgGAE/g), saponin (14.80%), tannin (0.11 mg/g) and DPPH (82.80%), has the highest flavonoid (0.33 mg RE/g), and alkaloid (20.50 mg/g) while, has the highest oxalate (77.50 mg/g). The GC-MS revealed significant chemical components of as 1-heptatriacotanol, germacra-1(10),4,11(13)-trien-12-oic acid, 6-alpha-hydroxy-,gamma-lactone, (E,E)-, 11H-Indeno [1,2b] quinoxaline, 2-methyl- while have butyl benzoate, benzoic acid, hexyl ester, hexadecanoic acid, methyl ester and have the following 15-hydroxypentadecanoic acid, -9-hexadecenal, and 11,13-dimethyl-12-tetradecen-1-ol acetate. All the produced toothpaste has a brown colour and a pleasant smell, with pH from 7.30 to 8.10 and foamability from 19.23% of stand-alone toothpaste to 44.44% of -based. Amongst the produced toothpaste -based toothpaste has the best antimicrobial activities against the tested bacteria (, ) and fungi ( and ). The stand-alone toothpaste has the lowest minimum inhibitory concentration of 1.25 mg/mL against bacteria and fungi.

Clinical Significance: The study provides information on the production of human health-friendly dental antimicrobial toothpaste from plant materials.