Background: Adenomyosis (AM) is a benign uterine disease characterized pathologically by the invasion of endometrial tissue into the myometrium. The pathogenesis of AM is still far from clear. Although the gut microbiome and metabolomics are thought to contribute to a variety of diseases, the role of them in AM has not been revealed.
Objective: To investigate changes in the gut microbiota and derived metabolites in AM mice.
Method: Female ICR mice were randomly assigned to AM and control groups, and pituitary transplantation was employed to perform AM modeling. Then, the fecal samples were obtained for microbial (16S rRNA gene sequencing) and metabolomic (liquid chromatography mass spectrometry, LC-MS) analysis.
Result: The results of gut microbiota analysis showed that the intestinal microbiota composition of AM mice was altered. The ratio of and the relative abundance of in AM group increased compared with the control group. Sixty differential expressed metabolites were identified in intestinal metabolites, mainly involved in steroid hormone biosynthesis, cysteine and methionine metabolism, and alanine, aspartate, and glutamate metabolism. Further, correlation analysis verified that -methionine and -cystine were negatively correlated with and positively correlated with . The Pregnenolone, Androsterone glucuronide, and Testosterone glucuronide were negatively correlated with and , whereas they positively correlated with .
Conclusion: AM mice have a unique gut microbiome and intestinal metabolites.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008959 | PMC |
| http://dx.doi.org/10.3389/fcimb.2023.1075387 | DOI Listing |
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