Unlabelled: Chimeric antigen receptor (CAR) T cells are efficacious in patients with B-cell malignancies, while their activity is limited in patients with solid tumors. We developed a novel heterodimeric TCR-like CAR (TCAR) designed to achieve optimal chain pairing and integration into the T-cell CD3 signaling complex. The TCAR mediated high antigen sensitivity and potent antigen-specific T-cell effector functions in short-term assays. Both persistence and functionality of TCAR T cells were augmented by provision of costimulatory signals, which improved proliferation and . Combination with a nanoparticulate RNA vaccine, developed for expansion of CAR T cells, promoted tightly controlled expansion, survival, and antitumor efficacy of TCAR T cells .
Significance: A novel TCAR is tightly controlled by RNA vaccine-mediated costimulation and may provide an alternative to second-generation CARs for the treatment of solid tumors.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10010320 | PMC |
| http://dx.doi.org/10.1158/2767-9764.CRC-21-0154 | DOI Listing |
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