Developing artificial symbionts beyond natural synthesis limitations would bring revolutionary contributions to agriculture, medicine, environment, etc. Here, we initiated a solar-driven multi-organism symbiont, which was assembled by the CO fixation module of sp., N fixation module of , biofunctional polypeptides synthesis module of , and the electron transfer module of conductive cationic poly(fluorene--phenylene) derivative. The modular design broke the pathway to synthesize γ-polyglutamic acid (γ-PGA) using CO and N, attributing to the artificially constructed direct interspecific substance and electron transfer. So, the intracellular ATP and NADPH were enhanced by 69 and 30%, respectively, and the produced γ-PGA was enhanced by 104%. The strategy was further extended to produce a commercial antibiotic of bacitracin A. These achievements improve the selectivity and yield of functional polypeptides with one click by CO and N, and also provide an innovative strategy for creating photosynthetic systems on demand.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017035 | PMC |
http://dx.doi.org/10.1126/sciadv.adf6772 | DOI Listing |
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