This study highlights the development of two lateral flow recombinase polymerase amplification assays for the diagnosis of human malaria. The lateral flow cassettes contained test lines that captured biotin-, 6-carboxyfluorescein, digoxigenin-, cyanine 5-, and dinitrophenyl-labeled amplicons. The overall process can be completed in 30 minutes. Recombinase polymerase amplification coupled with lateral flow had a detection limit of 1 copy/µL for Plasmodium knowlesi, Plasmodium vivax, and Plasmodium falciparum. No cross-reactivity was observed among nonhuman malaria parasites such as Plasmodium coatneyi, Plasmodium cynomolgi, Plasmodium brasilanium, Plasmodium inui, Plasmodium fragile, Toxoplasma gondii, Sarcocystis spp., Brugia spp., and 20 healthy donors. It is rapid, highly sensitive, robust, and easy to use. The result can be read without the need for special equipment and thus has the potential to serve as an effective alternative to polymerase chain reaction methods for the diagnosis of malaria.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10160889 | PMC |
| http://dx.doi.org/10.4269/ajtmh.22-0657 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Aflatoxin Contamination of Various Staple Foods from Angola and Mozambique.
Toxins (Basel)
November 2024
CIMO, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal.
Aflatoxins constitute a significant risk in staple foods produced in African countries. This research aimed to analyze the total aflatoxin (AFT) contamination of various staple foods in Angola and Mozambique. A total of 233 samples of corn, peanuts, beans, rice, and cassava flour collected from farmers or local markets from the province of Cuanza Sul, Angola, and the provinces of Gaza and Inhambane, South Mozambique, were analyzed for the presence of AFT using the lateral flow strip method via AgraStrip Pro WATEX (Romer).
View Article and Find Full Text PDFInfluence of Fungal Colonization on Exacerbations in Patients with Cystic Fibrosis.
J Fungi (Basel)
December 2024
Pneumology Department, Central Universitary Hospital of Asturias (HUCA), 33011 Oviedo, Spain.
The importance of fungal pathogens in cystic fibrosis (CF) patients and their diagnosis remains a challenge, so our aim was to analyze the influence of the detection of fungi in sputum by using conventional culture and molecular techniques, polymerase chain reaction (PCR), lateral flow devices (LFDs), and galactomannan (GM) on exacerbations in patients with cystic fibrosis. A prospective study was conducted in patients via follow-up in the CF Unit of the Central University Hospital of Asturias from January 2021 to April 2022. Adult patients with at least one documented exacerbation were included.
View Article and Find Full Text PDFCurrent Analytical Methods and Challenges for the Clinical Diagnosis of Invasive Pulmonary Aspergillosis Infection.
J Fungi (Basel)
November 2024
Department of Chemistry, Colorado State University, 1801 Campus Delivery, Fort Collins, CO 80523, USA.
In the last decade, pulmonary fungal infections such as invasive pulmonary aspergillosis (IPA) have increased in incidence due to the increased number of immunocompromised individuals. This increase is especially problematic when considering mortality rates associated with IPA are upwards of 70%. This high mortality rate is due to, in part, the length of time it takes to diagnose a patient with IPA.
View Article and Find Full Text PDFPaper-Based Aptasensor Assay for Detection of Food Adulterant Sildenafil.
Biosensors (Basel)
December 2024
Department of Medical Biology, School of Medicine, Atilim University, Ankara 06830, Turkey.
Sildenafil is used to treat erectile dysfunction and pulmonary arterial hypertension but is often illicitly added to energy drinks and chocolates. This study introduces a lateral flow strip test using aptamers specific to sildenafil for detecting its illegal presence in food. The process involved using graphene oxide SELEX to identify high-affinity aptamers, which were then converted into molecular gate structures on mesoporous silica nanoparticles, creating a unique signaling system.
View Article and Find Full Text PDFEnhancing Sensitivity of Point-of-Care Thyroid Diagnosis via Computational Analysis of Lateral Flow Assay Images Using Novel Textural Features and Hybrid-AI Models.
Biosensors (Basel)
December 2024
School of Engineering, Ulster University, Belfast BT15 1ED, UK.
Lateral flow assays are widely used in point-of-care diagnostics but face challenges in sensitivity and accuracy when detecting low analyte concentrations, such as thyroid-stimulating hormone biomarkers. This study aims to enhance assay performance by leveraging textural features and hybrid artificial intelligence models. A modified Gray-Level Co-occurrence Matrix, termed the Averaged Horizontal Multiple Offsets Gray-Level Co-occurrence Matrix, was utilised to compute the textural features of the biosensor assay images.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!