This article comments on: . 2023. Stress response requires an efficient glycogen and central carbon metabolism connection by phosphoglucomutases in cyanobacteria. Journal of Experimental Botany , 1532–1550
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| http://dx.doi.org/10.1093/jxb/erac513 | DOI Listing |
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Phosphoglucomutase comes into the spotlight.
J Exp Bot
March 2023
Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Germany.
This article comments on: . 2023. Stress response requires an efficient glycogen and central carbon metabolism connection by phosphoglucomutases in cyanobacteria.
View Article and Find Full Text PDFDemonstrating the utility of sugar-phosphate phosphatases in coupled enzyme assays: galactose-1-phosphate uridylyltransferase as proof-of-concept.
Glycobiology
March 2023
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
During our biochemical characterization of select bacterial phosphatases belonging to the haloacid dehalogenase superfamily of hydrolases, we discovered a strong bias of Salmonella YidA for glucose-1-phosphate (Glc-1-P) over galactose-1-phosphate (Gal-1-P). We sought to exploit this ability of YidA to discriminate these two sugar-phosphate epimers in a simple coupled assay that could be a substitute for current cumbersome alternatives. To this end, we focused on Gal-1-P uridylyltransferase (GalT) that is defective in individuals with classical galactosemia, an inborn disorder.
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