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Canine peripheral blood TCRαβ T cell atlas: Identification of diverse subsets including CD8A MAIT-like cells by combined single-cell transcriptome and V(D)J repertoire analysis. | LitMetric

AI Article Synopsis

  • Dogs are valuable companion animals and may provide insights into human disorders, particularly related to T cells, which play a crucial role in health and disease.
  • This study aimed to map out the types of TCRαβ T cells in healthy dogs using advanced techniques like single-cell RNA sequencing (scRNAseq) and immune repertoire sequencing, leading to the identification of 22 distinct T cell clusters.
  • The findings revealed previously unidentified T cell populations, including canine mucosa-associated invariant T cell-like cells, and provide a valuable resource for enhancing our understanding of canine immunology and developing targeted therapies.

Article Abstract

The dog is valued as a companion animal and increasingly recognized as a model for human disorders. Given the importance of T cells in health and disease, comprehensive knowledge of canine T cells can contribute to our understanding of pathogenesis mechanisms and inform the development of new treatment strategies. However, the diversity of canine T cells is still poorly understood mainly due to the lack of species-reactive antibodies for use in flow cytometry. The aim of this study was to generate a detailed atlas of peripheral blood TCRαβ T cells of healthy dogs using single-cell RNA-sequencing (scRNAseq) combined with immune repertoire sequencing. A total of 22 TCRαβ T cell clusters were identified, which were classified into three major groups: CD4-dominant (11 clusters), CD8A-dominant (8 clusters), and CD4/CD8A-mixed (3 clusters). Based on differential gene expression, distinct differentiation states (naïve, effector, memory, exhausted) and lineages (e.g. CD4 T helper and regulatory T cells) could be distinguished. Importantly, several T cell populations were identified, which have not been described in dogs before. Of particular note, our data provide first evidence for the existence of canine mucosa-associated invariant T cell (MAIT)-like cells, representing one of three newly identified FCER1G innate-like CD8A T cell populations in the peripheral blood of healthy dogs. In conclusion, using scRNAseq combined with immune repertoire sequencing we were able to resolve canine TCRαβ T cell populations at unprecedented resolution. The peripheral blood TCRαβ T cell atlas of healthy dogs generated here represents an important reference data set for future studies and is of relevance for identifying new targets for T cell-specific therapies.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995359PMC
http://dx.doi.org/10.3389/fimmu.2023.1123366DOI Listing

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