The most common intracellular bacterial infection is a microbe that manipulates host reproduction and is used in control of insect vectors. Phenotypes induced by have been studied for decades and range from sperm-egg incompatibility to male killing. How alters host biology is less well understood. Previously, we characterized the first effector - WalE1, which encodes a synuclein domain at the N terminus. Purified WalE1 sediments with and bundles actin and when heterologously expressed in flies, increases titer in the developing oocyte. In this work, we first identify the native expression WalE1 by infecting both fly cells and whole animals. WalE1 appears as aggregates, separate from cells. We next show that WalE1 co-immunoprecipitates with the host protein Past1 and that WalE1 manipulates host endocytosis. Yeast expressing WalE1 show deficiency in uptake of FM4-64 dye, and flies harboring mutations in or overexpressing WalE1 are sensitive to AgNO, a hallmark of endocytosis defects. Finally, we also show that null flies harbor more overall and in late egg chambers. Our results identify interactions between a secreted effector and a host protein and point to yet another important host cell process impinged upon by
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http://dx.doi.org/10.1101/2023.02.26.530160 | DOI Listing |
Microb Ecol
December 2024
Department of Biotechnology, Periyar University, Centre for Postgraduate and Research Studies, Dharmapuri, Tamil Nadu, 635205, India.
Mosquito-borne illnesses pose a significant threat to eradication under existing vector management measures. Chemo-based vector control strategies (use of insecticides) raise a complication of resistance and environmental pollution. Biological control methods are an alternative approach to overcoming this complication arising from insecticides.
View Article and Find Full Text PDFPLoS Pathog
March 2024
Department of Biology, Indiana University, Bloomington, Indiana United States of America.
The most common intracellular bacterial infection is Wolbachia pipientis, a microbe that manipulates host reproduction and is used in control of insect vectors. Phenotypes induced by Wolbachia have been studied for decades and range from sperm-egg incompatibility to male killing. How Wolbachia alters host biology is less well understood.
View Article and Find Full Text PDFbioRxiv
February 2023
Department of Biology, Indiana University, Bloomington, IN USA.
The most common intracellular bacterial infection is a microbe that manipulates host reproduction and is used in control of insect vectors. Phenotypes induced by have been studied for decades and range from sperm-egg incompatibility to male killing. How alters host biology is less well understood.
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