One-Pot Synthesis of Enzyme and Antibody/CaHPO Nanoflowers for Magnetic Chemiluminescence Immunoassay of .

In this study, through a bioinspired strategy, the horseradish peroxidase (HRP) and antibody (Ab) were co-embedded into CaHPO to prepare HRP-Ab-CaHPO (HAC) bifunctional hybrid nanoflowers by one-pot mild coprecipitation. The as-prepared HAC hybrid nanoflowers then were utilized as the signal tag in a magnetic chemiluminescence immunoassay for application in the detection of (). The proposed method exhibited excellent detection performance in the linear range of 10-10 CFU/mL, with the limit of detection (LOD) of 10 CFU/mL. This study indicates great potential in the sensitive detection of foodborne pathogenic bacteria in milk with this new magnetic chemiluminescence biosensing platform.