Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
There is no published information on the seed germination or seed storage physiology of , , and This lack of information is hampering conservation efforts of these critically endangered species. This study investigated the seed morphology, seed germination requirements, and long-term seed storage methods for all three species. The impact of desiccation, desiccation and freezing, as well as desiccation plus storage at 5 °C, -18 °C, and -196 °C on seed viability (germination) and seedling vigour was assessed. Fatty acid profiles were compared between and . Variability in storage behaviour between the three species was investigated through differential scanning calorimetry (DSC) by comparing thermal properties of lipids. seed were desiccation-tolerant and viability was retained when desiccated seed was stored for 24 months at 5 °C. seed was both desiccation- and freezing-sensitive, while was desiccation-sensitive. DSC analysis revealed that lipid crystallisation in occurred between -18 °C and -49 °C and between -23 °C and -52 °C in and . It is postulated that the metastable lipid phase, which coincides with the conventional seed banking temperature (i.e., storing seeds at -20 ± 4 °C and 15 ± 3% RH), could cause the seeds to age more rapidly through lipid peroxidation. Seeds of , and are best stored outside of their lipid metastable temperature ranges.
Download full-text PDF |
Source |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005796 | PMC |
http://dx.doi.org/10.3390/plants12051067 | DOI Listing |
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