AI Article Synopsis
- Long non-coding RNAs (lncRNAs) are important for regulating biological processes in plants, but their roles in kiwifruit ripening and softening are not well understood.
- In this study, researchers identified 591 differentially expressed lncRNAs and 3107 differentially expressed genes by comparing kiwifruit stored at 4 °C for 1, 2, and 3 weeks to untreated controls.
- The findings suggest that lncRNAs significantly influence fruit ripening and softening by regulating genes involved in starch and sucrose metabolism, as well as cell wall modification during low-temperature storage.
Article Abstract
Long non-coding RNAs (lncRNAs) are crucial players regulating many biological processes in plants. However, limited knowledge is available regarding their roles in kiwifruit ripening and softening. In this study, using lncRNA-seq technology, 591 differentially expressed (DE) lncRNAs (DELs) and 3107 DE genes (DEGs) were identified from kiwifruit stored at 4 °C for 1, 2, and 3 weeks in comparison with non-treated control fruits. Of note, 645 DEGs were predicted to be targets of DELs (DEGTLs), including some DE protein-coding genes (such as β-amylase and pectinesterase). DEGTL-based GO enrichment analysis revealed that these genes were significantly enriched in cell wall modification and pectinesterase activity in 1 W vs. CK and 3 W vs. CK, which might be closely related to the fruit softening during low-temperature storage. Moreover, KEGG enrichment analysis revealed that DEGTLs were significantly associated with starch and sucrose metabolism. Our study revealed that lncRNAs play critical regulatory roles in kiwifruit ripening and softening under low-temperature storage, mainly by mediating the expression of starch and sucrose metabolism and cell wall modification related genes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005093 | PMC |
| http://dx.doi.org/10.3390/plants12051070 | DOI Listing |
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