Binary Toxin Binding Component Increases Virulence in a Hamster Model.

Background: is the leading cause of hospital-acquired gastrointestinal infection, in part due to the existence of binary toxin (CDT)-expressing hypervirulent strains. Although the effects of the CDT holotoxin on disease pathogenesis have been previously studied, we sought to investigate the role of the individual components of CDT during in vivo infection.

Methods: To determine the contribution of the separate components of CDT during infection, we developed strains of expressing either CDTa or CDTb individually. We then infected both mice and hamsters with these novel mutant strains and monitored them for development of severe illness.

Results: Although expression of CDTb without CDTa did not induce significant disease in a mouse model of infection, we found that complementation of a CDT-deficient strain with CDTb alone restored virulence in a hamster model of infection.

Conclusions: Overall, this study demonstrates that the binding component of binary toxin, CDTb, contributes to virulence in a hamster model of infection.