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Inhibition of NHE1 transport activity and gene transcription in DRG neurons in oxaliplatin-induced painful peripheral neurotoxicity. | LitMetric

AI Article Synopsis

  • Oxaliplatin (OHP) causes peripheral neurotoxicity (OIPN), a significant side effect of colorectal cancer treatment, with both acute and chronic forms.
  • The study found that low doses of OHP reduce the pH recovery rate in dorsal root ganglion (DRG) neurons by inhibiting the Na/H exchanger isoform-1 (NHE1), which helps maintain intracellular pH.
  • OHP's effects on NHE1 activity involve calcineurin (CaN) mediation, and the downregulation of NHE1 in both cultured neurons and a rat model suggests new potential targets for improving treatment outcomes.

Article Abstract

Oxaliplatin (OHP)-induced peripheral neurotoxicity (OIPN), one of the major dose-limiting side effects of colorectal cancer treatment, is characterized by both acute and chronic syndromes. Acute exposure to low dose OHP on dorsal root ganglion (DRG) neurons is able to induce an increase in intracellular calcium and proton concentration, thus influencing ion channels activity and neuronal excitability. The Na/H exchanger isoform-1 (NHE1) is a plasma membrane protein that plays a pivotal role in intracellular pH (pH) homeostasis in many cell types, including nociceptors. Here we show that OHP has early effects on NHE1 activity in cultured mouse DRG neurons: the mean rate of pH recovery was strongly reduced compared to vehicle-treated controls, reaching levels similar to those obtained in the presence of cariporide (Car), a specific NHE1 antagonist. The effect of OHP on NHE1 activity was sensitive to FK506, a specific calcineurin (CaN) inhibitor. Lastly, molecular analyses revealed transcriptional downregulation of NHE1 both in vitro, in mouse primary DRG neurons, and in vivo, in an OIPN rat model. Altogether, these data suggest that OHP-induced intracellular acidification of DRG neurons largely depends on CaN-mediated NHE1 inhibition, revealing new mechanisms that OHP could exert to alter neuronal excitability, and providing novel druggable targets.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9998445PMC
http://dx.doi.org/10.1038/s41598-023-31095-9DOI Listing

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