Hypoxia preconditioned DPSC-derived exosomes regulate angiogenesis via transferring LOXL2.

Exp Cell Res

Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510080, China; Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China. Electronic address:

Published: April 2023

AI Article Synopsis

  • Hypoxia enhances the ability of dental pulp stem cells (DPSCs) to promote angiogenesis, but the underlying mechanism remains unclear, particularly regarding the role of exosomes derived from these stem cells.
  • The study investigates whether LOXL2, a protein upregulated by hypoxia in DPSC-derived exosomes (Hypo-Exos), plays a significant role in mediating angiogenesis.
  • Results show that silencing LOXL2 in DPSCs reduces their proliferation and migration, while also diminishing the angiogenic effects of Hypo-Exos on human umbilical vein endothelial cells (HUVECs), indicating LOXL2 is a contributing factor in the angiogenic potential of these exosomes.

Article Abstract

Hypoxia was proved to enhance the angiogenesis of stem cells. However, the mechanism of the angiogenic potential in hypoxia-pretreated dental pulp stem cells (DPSCs) is poorly understood. We previously confirmed that hypoxia enhances the angiogenic potential of DPSC-derived exosomes with upregulation of lysyl oxidase-like 2 (LOXL2). Therefore, our study aimed to illuminate whether these exosomes promote angiogenesis via transfer of LOXL2. Exosomes were generated from hypoxia-pretreated DPSCs (Hypo-Exos) stably silencing LOXL2 after lentiviral transfection and characterized with transmission electron microscopy, nanosight and Western blot. The efficiency of silencing was verified using quantitative real-time PCR (qRT-PCR) and Western blot. CCK-8, scratch and transwell assays were conducted to explore the effects of LOXL2 silencing on DPSCs proliferation and migration. Human umbilical vein endothelial cells (HUVECs) were co-incubated with exosomes to assess the migration and angiogenic capacity through transwell and matrigel tube formation assays. The relative expression of angiogenesis-associated genes was characterized by qRT-PCR and Western blot. LOXL2 was successfully silenced in DPSCs and inhibited DPSC proliferation and migration. LOXL2 silencing in Hypo-Exos partially reduced promotion of HUVEC migration and tube formation and inhibited the expression of angiogenesis-associated genes. Thus, LOXL2 is one of various factors mediating the angiogenic effects of Hypo-Exos.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.yexcr.2023.113543DOI Listing

Publication Analysis

Top Keywords

western blot
12
dpsc-derived exosomes
8
loxl2
8
stem cells
8
angiogenic potential
8
qrt-pcr western
8
loxl2 silencing
8
proliferation migration
8
tube formation
8
expression angiogenesis-associated
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!